MOBKL1A polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MOBKL1A.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MOBKL1A.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MOBKL1A polyclonal antibody (Cat # PAB4556) in Ramos cell line lysates (35 ug/lane). MOBKL1A (arrow) was detected using the purified MOBKL1A polyclonal antibody (Cat # PAB4556).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with MOBKL1A polyclonal antibody (Cat # PAB4556) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .Enzyme-linked Immunoabsorbent Assay
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Gene Info — MOBKL1A
Entrez GeneID
92597Protein Accession#
NP_775739;Q7L9L4Gene Name
MOBKL1A
Gene Alias
MATS2, MGC33910, MOB4A, Mob1B
Gene Description
MOB1, Mps One Binder kinase activator-like 1A (yeast)
Omim ID
609282Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. [provided by RefSeq
Other Designations
MOB1, Mps One Binder kinase activator-like 1A
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Interactome
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Publication Reference
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Mechanism of activation of NDR (nuclear Dbf2-related) protein kinase by the hMOB1 protein.
Bichsel SJ, Tamaskovic R, Stegert MR, Hemmings BA.
The Journal of Biological Chemistry 2004 Aug; 279(34):35228.
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Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases.
Devroe E, Erdjument-Bromage H, Tempst P, Silver PA.
The Journal of Biological Chemistry 2004 Apr; 279(23):24444.
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Mechanism of activation of NDR (nuclear Dbf2-related) protein kinase by the hMOB1 protein.
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