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Last updated: 2016/12/4
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PRKD3 polyclonal antibody

  • Catalog # : PAB4545
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PRKD3.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PRKD3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:100-500)
    Immunohistochemistry (1:50-100)
    Immunofluorescence (1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of PRKD3 polyclonal antibody (Cat # PAB4545) in NCI-H460 cell lysate . PRKD3 (arrow) was detected using purified PRKD3 polyclonal antibody (Cat # PAB4545) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using an PRKD3 polyclonal antibody (Cat # PAB4545) .
    Lanes 1 and 5 : non-transfected cells;
    lanes 2 and 6 : GFP-PRKD-transfected cells;
    lanes 3 and 7 : GFP-PRKD2-transfected cells;
    lanes 4 and 8 : GFP-PRKD3 transfected cells .
    Lower panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using PRKD3 polyclonal antibody (Cat # PAB4544) and PRKD3 polyclonal antibody (Cat # PAB4545) .
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the PRKD3 polyclonal antibody (Cat # PAB4545) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
  • Immunofluorescence
  • Immunofluorescence
  • Indirect immunofluorescence analysis of GFP-PRKD3 fusion protein expression in Panc-1 cells by using PRKD3 polyclonal antibody (Cat # PAB4544) and PRKD3 polyclonal antibody (Cat # PAB4545) . Data courtesy of Dr . Osvaldo Rey, University of California Los Angeles .
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • PRKD3
  • Gene Alias:
  • EPK2,PKC-NU,PKD3,PRKCN,nPKC-NU
  • Gene Description:
  • protein kinase D3
  • Gene Summary:
  • Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000126953,protein kinase C, nu,protein kinase EPK2,protein-serine/threonine kinase
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