PRKD1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of PRKD1 polyclonal antibody (Cat # PAB4541) in mouse kidney lysate . PRKD1 (arrow) was detected using purified PRKD1 polyclonal antibody (Cat # PAB4541) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKD1 polyclonal antibody (Cat # PAB4541) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKD1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PRKD1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of PRKD1 polyclonal antibody (Cat # PAB4541) in mouse kidney lysate . PRKD1 (arrow) was detected using purified PRKD1 polyclonal antibody (Cat # PAB4541) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKD1 polyclonal antibody (Cat # PAB4541) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKD1
Entrez GeneID
5587Protein Accession#
NP_002733;Q15139Gene Name
PRKD1
Gene Alias
PKC-MU, PKCM, PKD, PRKCM
Gene Description
protein kinase D1
Omim ID
605435Gene Ontology
HyperlinkGene Summary
Members of the protein kinase C (PKC) family function in many extracellular receptor-mediated signal transduction pathways. See PRKCA (MIM 176960) for further background information. The PRKCM gene encodes a cytosolic serine-threonine kinase that binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface.[supplied by OMIM
Other Designations
protein kinase C, mu
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Interactome
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Disease
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Publication Reference
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Protein kinase C zeta. A novel regulator of both phosphorylation and de-phosphorylation of cardiac sarcomeric proteins.
Wu SC, Solaro RJ.
The Journal of Biological Chemistry 2007 Aug; 282(42):30691.
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Protein kinase C zeta. A novel regulator of both phosphorylation and de-phosphorylation of cardiac sarcomeric proteins.
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