Products

Western Blot (Tissue lysate)

Western blot analysis of PRKCI polyclonal antibody (Cat # PAB4540) in mouse lung lysate . PRKCI (arrow) was detected using purified PRKCI polyclonal antibody (Cat # PAB4540) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .

Western Blot (Transfected lysate)

Western blot analysis of PRKCI (arrow) using rabbit PRKCI polyclonal antibody (Cat # PAB4540). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCI gene (Lane 2) (Origene Technologies).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PRKCI polyclonal antibody (Cat # PAB4540) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

5584

Protein Accession#

P41743

Gene Name

PRKCI

Gene Alias

DXS1179E, MGC26534, PKCI, nPKC-iota

Gene Description

protein kinase C, iota

Omim ID

600539

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq

Other Designations

OTTHUMP00000196630|PRKC-lambda/iota|aPKC-lambda/iota|atypical protein kinase C-lambda/iota|protein kinase C iota type