PRKCH polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKCH.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PRKCH.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PRKCH polyclonal antibody (Cat # PAB4538) in NCI-H460 cell lysate . PRKCH (arrow) was detected using purified PRKCH polyclonal antibody (Cat # PAB4538) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKCH polyclonal antibody (Cat # PAB4538) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKCH
Entrez GeneID
5583Protein Accession#
P24723Gene Name
PRKCH
Gene Alias
MGC26269, MGC5363, PKC-L, PKCL, PRKCL, nPKC-eta
Gene Description
protein kinase C, eta
Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. [provided by RefSeq
Other Designations
protein kinase C eta type
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of multiple, novel, protein kinase C-related gene products.
Palmer RH, Ridden J, Parker PJ.
FEBS Letters 1994 Dec; 356(1):5.
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Isolation and characterization of PKC-L, a new member of the protein kinase C-related gene family specifically expressed in lung, skin, and heart.
Bacher N, Zisman Y, Berent E, Livneh E.
Molecular and Cellular Biology 1992 Mar; 12(3):1404.
Application:WB-Tr, Monkey, COS cells.
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Identification of multiple, novel, protein kinase C-related gene products.
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