PRKCE polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of PRKCE polyclonal antibody (Cat # PAB4537) in placenta lysate . PRKCE (Arrow) was detected using PRKCE polyclonal antibody (Cat # PAB4537) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Western Blot (Transfected lysate)
Western blot analysis of PRKCE (arrow) using rabbit PRKCE polyclonal antibody (Cat # PAB4537). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCE gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PRKCE polyclonal antibody (Cat # PAB4537) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKCE.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 213-243 at N-terminus of human PRKCE.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of PRKCE polyclonal antibody (Cat # PAB4537) in placenta lysate . PRKCE (Arrow) was detected using PRKCE polyclonal antibody (Cat # PAB4537) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .Western Blot (Transfected lysate)
Western blot analysis of PRKCE (arrow) using rabbit PRKCE polyclonal antibody (Cat # PAB4537). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCE gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PRKCE polyclonal antibody (Cat # PAB4537) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKCE
Entrez GeneID
5581Protein Accession#
Q02156Gene Name
PRKCE
Gene Alias
MGC125656, MGC125657, PKCE, nPKC-epsilon
Gene Description
protein kinase C, epsilon
Omim ID
176975Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq
Other Designations
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Publication Reference
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Activated PKC{delta} and PKC{epsilon} inhibit epithelial chloride secretion response to cAMP via inducing internalization of the Na+-K+-2Cl- cotransporter NKCC1.
Tang J, Bouyer P, Mykoniatis A, Buschmann M, Matlin KS, Matthews JB.
The Journal of Biological Chemistry 2010 Oct; 285(44):34072.
Application：IF, Human, T84 cells.
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Induction of neurites by the regulatory domains of PKCdelta and epsilon is counteracted by PKC catalytic activity and by the RhoA pathway.
Ling M, Troller U, Zeidman R, Lundberg C, Larsson C.
Experimental Cell Research 2004 Jan; 292(1):135.
Application：WB, Human, HiB5 cells.
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Preservation of base-line hemodynamic function and loss of inducible cardioprotection in adult mice lacking protein kinase C epsilon.
Gray MO, Zhou HZ, Schafhalter-Zoppoth I, Zhu P, Mochly-Rosen D, Messing RO.
The Journal of Biological Chemistry 2004 Jan; 279(5):3596.
Application：WB-Ti, Mouse, Mouse hearts.
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Protein kinase Cepsilon interacts with Bax and promotes survival of human prostate cancer cells.
McJilton MA, Van Sikes C, Wescott GG, Wu D, Foreman TL, Gregory CW, Weidner DA, Harris Ford O, Morgan Lasater A, Mohler JL, Terrian DM.
Oncogene 2003 Sep; 22(39):7958.
Application：IHC-P, IP, WB, Human, CWR-R1, LNCaP cells, Prostate tissues.
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Activated PKC{delta} and PKC{epsilon} inhibit epithelial chloride secretion response to cAMP via inducing internalization of the Na+-K+-2Cl- cotransporter NKCC1.
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