PRKCD polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of PRKCD polyclonal antibody (Cat # PAB4536) in placenta lysate . PRKCD delta (Arrow) was detected using purified PRKCD polyclonal antibody (Cat # PAB4536) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Western Blot (Transfected lysate)
Western blot analysis of PRKCD (arrow) using rabbit PRKCD polyclonal antibody (Cat # PAB4536). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCD gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKCD polyclonal antibody (Cat # PAB4536) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKCD.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PRKCD.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of PRKCD polyclonal antibody (Cat # PAB4536) in placenta lysate . PRKCD delta (Arrow) was detected using purified PRKCD polyclonal antibody (Cat # PAB4536) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .Western Blot (Transfected lysate)
Western blot analysis of PRKCD (arrow) using rabbit PRKCD polyclonal antibody (Cat # PAB4536). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCD gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKCD polyclonal antibody (Cat # PAB4536) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKCD
Entrez GeneID
5580Protein Accession#
Q05655Gene Name
PRKCD
Gene Alias
MAY1, MGC49908, PKCD, nPKC-delta
Gene Description
protein kinase C, delta
Omim ID
176977Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
protein kinase C delta VIII
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Interactome
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Pathway
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Disease
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Publication Reference
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Functional analyses of mammalian protein kinase C isozymes in budding yeast and mammalian fibroblasts.
Nomoto S, Watanabe Y, Ninomiya-Tsuji J, Yang LX, Nagai Y, Kiuchi K, Hagiwara M, Hidaka H, Matsumoto K, Irie K.
Genes to Cells 1997 Oct; 2(10):601.
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Molecular and biochemical characterization of a recombinant human PKC-delta family member.
Aris JP, Basta PV, Holmes WD, Ballas LM, Moomaw C, Rankl NB, Blobel G, Loomis CR, Burns DJ.
Biochimica et Biophysica Acta 1993 Aug; 1174(2):171.
Application：WB-Tr, Insect, Sf9 cells.
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Functional analyses of mammalian protein kinase C isozymes in budding yeast and mammalian fibroblasts.
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