Western blot analysis of PRKCD polyclonal antibody (Cat # PAB4536) in placenta lysate . PRKCD delta (Arrow) was detected using purified PRKCD polyclonal antibody (Cat # PAB4536) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Western Blot (Transfected lysate)
Western blot analysis of PRKCD (arrow) using rabbit PRKCD polyclonal antibody (Cat # PAB4536). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCD gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKCD polyclonal antibody (Cat # PAB4536) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq