Western blot analysis of PRKCA polyclonal antibody (Cat # PAB4531) in placenta lysate . PRKCA (arrow) was detected using purified PRKCA polyclonal antibody (Cat # PAB4531) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKCA polyclonal antibody (Cat # PAB4531) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq
aging-associated gene 6,protein kinase C alpha type