PRKACA polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKACA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PRKACA.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The PRKACA polyclonal antibody (Cat # PAB4529) is used in Western blot to detect PRKACA alpha in mouse heart tissue lysate .Western Blot (Cell lysate)
The PRKACA polyclonal antibody (Cat # PAB4529) is used in Western blot to detect PRKACA alpha in Jurkat cell lysate .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PRKACA polyclonal antibody (Cat # PAB4529) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKACA
Entrez GeneID
5566Protein Accession#
P17612Gene Name
PRKACA
Gene Alias
MGC102831, MGC48865, PKACA
Gene Description
protein kinase, cAMP-dependent, catalytic, alpha
Omim ID
601639Gene Ontology
HyperlinkGene Summary
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
PKA C-alpha|cAMP-dependent protein kinase catalytic subunit alpha|cAMP-dependent protein kinase catalytic subunit alpha, isoform 1|protein kinase A catalytic subunit
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Interactome
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Pathway
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Publication Reference
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Modulation of cardiac sodium channel gating by protein kinase A can be altered by disease-linked mutation.
Tateyama M, Rivolta I, Clancy CE, Kass RS.
The Journal of Biological Chemistry 2003 Nov; 278(47):46718.
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Active cAMP-dependent protein kinase incorporated within highly purified HIV-1 particles is required for viral infectivity and interacts with viral capsid protein.
Cartier C, Hemonnot B, Gay B, Bardy M, Sanchiz C, Devaux C, Briant L.
The Journal of Biological Chemistry 2003 Sep; 278(37):35211.
Application:IEM, WB, Human, H9 cells.
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Differential regulation of P-selectin expression by protein kinase A and protein kinase G in thrombin-stimulated human platelets.
Libersan D, Rousseau G, Merhi Y.
Thrombosis and Haemostasis 2003 Feb; 89(2):310.
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Modulation of cardiac sodium channel gating by protein kinase A can be altered by disease-linked mutation.
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