GRK6 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GRK6.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human GRK6.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
Western blot analysis of GRK6 polyclonal antibody (Cat # PAB4520) in mouse heart tissue lysate . GRK6 (arrow) was detected using purified GRK6 polyclonal antibody (Cat # PAB4520) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .Western Blot (Cell lysate)
Western blot analysis of GRK6 polyclonal antibody (Cat # PAB4520) in HeLa cell lysate . GRK6 (arrow) was detected using purified GRK6 polyclonal antibody (Cat # PAB4520) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with GRK6 polyclonal antibody (Cat # PAB4520) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
-
Gene Info — GRK6
Entrez GeneID
2870Protein Accession#
P43250Gene Name
GRK6
Gene Alias
FLJ32135, GPRK6
Gene Description
G protein-coupled receptor kinase 6
Omim ID
600869Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq
Other Designations
-
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA,
PNAS 2002 Dec; 99(26):16899.
-
Palmitoylation of G protein-coupled receptor kinase, GRK6. Lipid modification diversity in the GRK family.
Stoffel RH, Randall RR, Premont RT, Lefkowitz RJ, Inglese J.
The Journal of Biological Chemistry 1994 Nov; 269(45):27791.
Application:IP, Insect, COS-7 cells, Sf9 cells, Recombinant proteins.
-
Molecular cloning and expression of GRK6. A new member of the G protein-coupled receptor kinase family.
Benovic JL, Gomez J.
The Journal of Biological Chemistry 1993 Sep; 268(26):19521.
-
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com