GPR37 polyclonal antibody
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More Files
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GPR37.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human GPR37.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of GPR37 polyclonal antibody (Cat # PAB4493) in mouse brain tissue lysate . GPR37 (arrow) was detected using the purified GPR37 polyclonal antibody (Cat # PAB4493) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with GPR37 polyclonal antibody (Cat # PAB4493) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — GPR37
Entrez GeneID
2861Protein Accession#
NP_005293.1Gene Name
GPR37
Gene Alias
EDNRBL, PAELR, hET(B)R-LP
Gene Description
G protein-coupled receptor 37 (endothelin receptor type B-like)
Omim ID
602583Gene Ontology
HyperlinkOther Designations
G protein-coupled receptor 37|Parkin-associated endothelin receptor-like receptor|endothelin receptor type B-like
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Interactomes
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Diseases
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Publication Reference
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Parkin suppresses dopaminergic neuron-selective neurotoxicity induced by Pael-R in Drosophila.
Yang Y, Nishimura I, Imai Y, Takahashi R, Lu B.
Neuron 2003 Mar; 37(6):911.
Application:IF, WB, Drosophila, Drosophila neuronal cultures.
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CHIP is associated with Parkin, a gene responsible for familial Parkinson's disease, and enhances its ubiquitin ligase activity.
Imai Y, Soda M, Hatakeyama S, Akagi T, Hashikawa T, Nakayama KI, Takahashi R.
Molecular Cell 2002 Jul; 10(1):55.
Application:IP, WB-Tr, Human, Brains, HEK 293T, SH-SY5Y cells.
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An unfolded putative transmembrane polypeptide, which can lead to endoplasmic reticulum stress, is a substrate of Parkin.
Imai Y, Soda M, Inoue H, Hattori N, Mizuno Y, Takahashi R.
Cell 2001 Jun; 105(7):891.
Application:IF, IP, WB-Tr, Human, Mouse, Brain, SH-SY5Y cells.
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Parkin suppresses dopaminergic neuron-selective neurotoxicity induced by Pael-R in Drosophila.
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