TSC2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TSC2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding S1798 of human TSC2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Ramos cell lysate (35 ug/lane) with TSC2 polyclonal antibody (Cat # PAB4446).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with TSC2 polyclonal antibody (Cat # PAB4446) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .Enzyme-linked Immunoabsorbent Assay
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Gene Info — TSC2
Entrez GeneID
7249Protein Accession#
NP_000539;P49815Gene Name
TSC2
Gene Alias
FLJ43106, LAM, TSC4
Gene Description
tuberous sclerosis 2
Gene Ontology
HyperlinkGene Summary
Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
tuberin
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Interactome
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Pathway
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Disease
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Publication Reference
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Biochemical and functional characterizations of small GTPase Rheb and TSC2 GAP activity.
Li Y, Inoki K, Guan KL.
Molecular and Cellular Biology 2004 Sep; 24(18):7965.
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Regulation of the TSC pathway by LKB1: evidence of a molecular link between tuberous sclerosis complex and Peutz-Jeghers syndrome.
Corradetti MN, Inoki K, Bardeesy N, DePinho RA, Guan KL.
Genes & Development 2004 Jul; 18(13):1533.
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Regulation of B-Raf kinase activity by tuberin and Rheb is mammalian target of rapamycin (mTOR)-independent.
Karbowniczek M, Cash T, Cheung M, Robertson GP, Astrinidis A, Henske EP.
The Journal of Biological Chemistry 2004 May; 279(29):29930.
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Biochemical and functional characterizations of small GTPase Rheb and TSC2 GAP activity.
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