GPC3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse stomach tissue lysate (35 ug/lane) with GPC3 polyclonal antibody (Cat # PAB4434).
Western Blot (Cell lysate)
Western blot analysis of HepG2 cell lysate (35 ug/lane) with GPC3 polyclonal antibody (Cat # PAB4434).
Immunofluorescence
Immunofluorecence staining of GPC3 polyclonal antibody (Cat # PAB4434) on HepG2 cells . The cells were acetone fixated . Antibody dilution of 1 : 50 . Original magnification 1 : 400 . Data and protocol courtesy of Dr . Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GPC3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human GPC3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse stomach tissue lysate (35 ug/lane) with GPC3 polyclonal antibody (Cat # PAB4434).Western Blot (Cell lysate)
Western blot analysis of HepG2 cell lysate (35 ug/lane) with GPC3 polyclonal antibody (Cat # PAB4434).Immunofluorescence
Immunofluorecence staining of GPC3 polyclonal antibody (Cat # PAB4434) on HepG2 cells . The cells were acetone fixated . Antibody dilution of 1 : 50 . Original magnification 1 : 400 . Data and protocol courtesy of Dr . Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine . -
Gene Info — GPC3
Entrez GeneID
2719Protein Accession#
NP_004475;P51654Gene Name
GPC3
Gene Alias
DGSX, OCI-5, SDYS, SGB, SGBS, SGBS1
Gene Description
glypican 3
Gene Ontology
HyperlinkGene Summary
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000024058|OTTHUMP00000062492|glypican proteoglycan 3
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Interactome
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Publication Reference
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Identification of glypican-3 as a novel tumor marker for melanoma.
Nakatsura T, Kageshita T, Ito S, Wakamatsu K, Monji M, Ikuta Y, Senju S, Ono T, Nishimura Y.
Clinical Cancer Research 2004 Oct; 10(19):6612.
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Methylation analysis of the glypican 3 gene in embryonal tumours.
Boily G, Saikali Z, Sinnett D.
British Journal of Cancer 2004 Apr; 90(8):1606.
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Glypican-3 is involved in cellular protection against mitoxantrone in gastric carcinoma cells.
Wichert A, Stege A, Midorikawa Y, Holm PS, Lage H.
Oncogene 2004 Jan; 23(4):945.
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Identification of glypican-3 as a novel tumor marker for melanoma.
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