NUB1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The NUB1 polyclonal antibody (Cat # PAB4349) is used in Western blot to detect NUB1 in mouse liver lysate .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NUB1 polyclonal antibody (Cat # PAB4349) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NUB1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human NUB1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The NUB1 polyclonal antibody (Cat # PAB4349) is used in Western blot to detect NUB1 in mouse liver lysate .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NUB1 polyclonal antibody (Cat # PAB4349) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — NUB1
Entrez GeneID
51667Protein Accession#
NP_057202;Q9Y5A7Gene Name
NUB1
Gene Alias
BS4, NUB1L, NYREN18
Gene Description
negative regulator of ubiquitin-like proteins 1
Omim ID
607981Gene Ontology
HyperlinkGene Summary
NUB1 interacts with and negatively regulates NEDD8 (MIM 603171), a ubiquitin-like protein that covalently conjugates to cullin (see MIM 603134) family members.[supplied by OMIM
Other Designations
NEDD8 ultimate buster-1|NY-REN-18 antigen
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Interactome
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Publication Reference
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Regulation of the NEDD8 conjugation system by a splicing variant, NUB1L.
Tanaka T, Kawashima H, Yeh ET, Kamitani T.
The Journal of Biological Chemistry 2003 Aug; 278(35):32905.
Application：WB-Tr, Yeast, AH109 yeast cells.
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NUB1, a NEDD8-interacting protein, is induced by interferon and down-regulates the NEDD8 expression.
Kito K, Yeh ET, Kamitani T.
The Journal of Biological Chemistry 2001 Jun; 276(23):20603.
Application：WB-Ce, Human, HeLa, 293, U2OS.
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Regulation of the NEDD8 conjugation system by a splicing variant, NUB1L.
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