CAPRIN1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The CAPRIN1 polyclonal antibody (Cat # PAB4337) is used in Western blot to detect CAPRIN1 in HL-60 cell lysate .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with CAPRIN1 polyclonal antibody (Cat # PAB4337) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CAPRIN1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 625-654 at C-terminus of human CAPRIN1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The CAPRIN1 polyclonal antibody (Cat # PAB4337) is used in Western blot to detect CAPRIN1 in HL-60 cell lysate .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with CAPRIN1 polyclonal antibody (Cat # PAB4337) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — CAPRIN1
Entrez GeneID
4076Protein Accession#
NP_976240;Q14444Gene Name
CAPRIN1
Gene Alias
GPIAP1, GPIP137, M11S1, caprin-1, p137GPI
Gene Description
cell cycle associated protein 1
Omim ID
601178Gene Ontology
HyperlinkGene Summary
chromosome 11
Other Designations
GPI-anchored membrane protein 1|GPI-anchored protein p137|activation/proliferation-associated protein 1|caprin 1|cytoplasmic activation/proliferation-associated protein-1|membrane component chromosome 11 surface marker 1|membrane component, chromosome 11,
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Interactome
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Publication Reference
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The gene encoding the GPI-anchored membrane protein p137GPI (M11S1) maps to human chromosome 11p13 and is highly conserved in the mouse.
Gessler M, Klamt B, Tsaoussidou S, Ellis JA, Luzio JP.
Genomics 1996 Feb; 32(1):169.
Application：WB-Ce, WB-Tr, Mouse, Mammalian cells.
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Identification and characterization of a novel protein (p137) which transcytoses bidirectionally in Caco-2 cells.
Ellis JA, Luzio JP.
The Journal of Biological Chemistry 1995 Sep; 270(35):20717.
Application：IP, WB-Ce, Human, Caco-2 cells.
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The gene encoding the GPI-anchored membrane protein p137GPI (M11S1) maps to human chromosome 11p13 and is highly conserved in the mouse.
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