MGAT3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MGAT3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human MGAT3.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse spleen tissue lysate (35 ug/lane) with MGAT3 polyclonal antibody (Cat # PAB4313).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MGAT3 polyclonal antibody (Cat # PAB4313) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — MGAT3
Entrez GeneID
4248Protein Accession#
GNT3_HUMANGene Name
MGAT3
Gene Alias
FLJ43371, GNT-III, GNT3, MGC141943, MGC142278
Gene Description
mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
Omim ID
604621Gene Ontology
HyperlinkGene Summary
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
GlcNAc-T III|N-acetylglucosaminyltransferase III|N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III|OTTHUMP00000028574|OTTHUMP00000042207|beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
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Interactome
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Pathway
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Publication Reference
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Human N-acetylglucosaminyltransferase III gene is transcribed from multiple promoters.
Koyama N, Miyoshi E, Ihara Y, Kang R, Nishikawa A, Taniguchi N.
European Journal of Biochemistry 1996 Jun; 238(3):853.
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Sequence analysis of the 5'-flanking region of the gene encoding human N-acetylglucosaminyltransferase III.
Kim YJ, Park JH, Kim KS, Chang JE, Ko JH, Kim MH, Chung DH, Chung TW, Choe IS, Lee YC, Kim CH.
Gene 1996 May; 170(2):281.
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Human N-acetylglucosaminyltransferase III gene is transcribed from multiple promoters.
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