RPN1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The RPN1 polyclonal antibody (Cat # PAB4310) is used in Western blot to detect RPN1 in mouse liver tissue lysate .
Western Blot (Cell lysate)
The RPN1 polyclonal antibody (Cat # PAB4310) is used in Western blot to detect RPN1 in HeLa cell lysate .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RPN1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human RPN1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The RPN1 polyclonal antibody (Cat # PAB4310) is used in Western blot to detect RPN1 in mouse liver tissue lysate .Western Blot (Cell lysate)
The RPN1 polyclonal antibody (Cat # PAB4310) is used in Western blot to detect RPN1 in HeLa cell lysate .Enzyme-linked Immunoabsorbent Assay
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Gene Info — RPN1
Entrez GeneID
6184Protein Accession#
P04843Gene Name
RPN1
Gene Alias
DKFZp686B16177, OST1, RBPH1
Gene Description
ribophorin I
Omim ID
180470Gene Ontology
HyperlinkGene Summary
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq
Other Designations
dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit
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Interactome
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Pathway
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Disease
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Publication Reference
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Retention of subunits of the oligosaccharyltransferase complex in the endoplasmic reticulum.
Fu J, Kreibich G.
The Journal of Biological Chemistry 2000 Feb; 275(6):3984.
Application：IF, Human, HeLa cells.
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Activation of a novel gene in 3q21 and identification of intergenic fusion transcripts with ecotropic viral insertion site I in leukemia.
Pekarsky Y, Rynditch A, Wieser R, Fonatsch C, Gardiner K.
Cancer Research 1997 Sep; 57(18):3914.
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Human ribophorins I and II: the primary structure and membrane topology of two highly conserved rough endoplasmic reticulum-specific glycoproteins.
Crimaudo C, Hortsch M, Gausepohl H, Meyer DI.
The EMBO Journal 1987 Jan; 6(1):75.
Application：WB-Ti, Dog, Pancreatic.
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Retention of subunits of the oligosaccharyltransferase complex in the endoplasmic reticulum.
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