GCNT1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of GCNT1 polyclonal antibody (Cat # PAB4302) in CEM cell line lysates (35 ug/lane). GCNT1 (arrow) was detected using the purified GCNT1 polyclonal antibody (Cat # PAB4302).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with GCNT1 polyclonal antibody (Cat # PAB4302) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GCNT1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human GCNT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of GCNT1 polyclonal antibody (Cat # PAB4302) in CEM cell line lysates (35 ug/lane). GCNT1 (arrow) was detected using the purified GCNT1 polyclonal antibody (Cat # PAB4302).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with GCNT1 polyclonal antibody (Cat # PAB4302) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — GCNT1
Entrez GeneID
2650Protein Accession#
G6NT_HUMANGene Name
GCNT1
Gene Alias
C2GNT, C2GNT-L, C2GNT1, G6NT, MGC126335, MGC126336, NACGT2, NAGCT2
Gene Description
glucosaminyl (N-acetyl) transferase 1, core 2 (beta-1,6-N-acetylglucosaminyltransferase)
Omim ID
600391Gene Ontology
HyperlinkGene Summary
This gene is a member of the beta-1,6-N-acetylglucosaminyltransferase gene family. It is essential to the formation of Gal beta 1-3(GlcNAc beta 1-6)GalNAc structures and the core 2 O-glycan branch. The gene coding this enzyme was originally mapped to 9q21, but was later localized to 9q13. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000021502|beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase|beta-1,6-N-acetylglucosaminyltransferase|core 2 GnT|core 2 beta-1,6-N-acetylglucosaminyltransferase I|core 2 branching enzyme
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Interactome
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Pathway
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Publication Reference
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Genomic organization of core 2 and I branching beta-1,6-N-acetylglucosaminyltransferases. Implication for evolution of the beta-1,6-N-acetylglucosaminyltransferase gene family.
Bierhuizen MF, Maemura K, Kudo S, Fukuda M.
Glycobiology 1995 Jun; 5(4):417.
Application：WB-Ce, WB-Tr, Human, Mammalian cells.
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Expression cloning of a cDNA encoding UDP-GlcNAc:Gal beta 1-3-GalNAc-R (GlcNAc to GalNAc) beta 1-6GlcNAc transferase by gene transfer into CHO cells expressing polyoma large tumor antigen.
Bierhuizen MF, Fukuda M.
PNAS 1992 Oct; 89(19):9326.
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Genomic organization of core 2 and I branching beta-1,6-N-acetylglucosaminyltransferases. Implication for evolution of the beta-1,6-N-acetylglucosaminyltransferase gene family.
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