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Last updated: 2021/1/10
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AGL polyclonal antibody 

  • Catalog # : PAB4298
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of AGL.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 1479-1510 at the C-terminus of human AGL.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • ELISA
    Immunofluorescence (1:10-50)
    Western Blot (1:8000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using AGL polyclonal antibody (Cat # PAB4298) at 1 : 500 dilution . A total of 20 ug of lysates was loaded for each tissue . Data courtesy of Dr . Alan Cheng, Department of Internal Medicine, Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, Michigan .
  • Immunofluorescence
  • Immunofluorescence
  • Expression of myc-GS causes wild type but not the CBD mutant of AGL to aggregate around the PAS-stain-positive inclusions. HepG2 cells were transfected with either HA-tagged wild-type AGL (HA-AGL) or HA-AGL CBD. Cells were fixed in formalin and processed for IF using anti-HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 178
  • Protein Accession#:
  • NP_000019;P35573
  • Gene Name:
  • AGL
  • Gene Alias:
  • GDE
  • Gene Description:
  • amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
  • Gene Summary:
  • This gene encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000012500,OTTHUMP00000012501,OTTHUMP00000012502,OTTHUMP00000012503,OTTHUMP00000012504,OTTHUMP00000012505,glycogen debranching enzyme
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