METAP2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of 293 cell lysate (35 ug/lane) with METAP2 polyclonal antibody (Cat # PAB4290).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with METAP2 polyclonal antibody (Cat # PAB4290) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of METAP2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human METAP2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 293 cell lysate (35 ug/lane) with METAP2 polyclonal antibody (Cat # PAB4290).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with METAP2 polyclonal antibody (Cat # PAB4290) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .Enzyme-linked Immunoabsorbent Assay
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Gene Info — METAP2
Entrez GeneID
10988Protein Accession#
AMP2_HUMANGene Name
METAP2
Gene Alias
MAP2, MNPEP, p67, p67eIF2
Gene Description
methionyl aminopeptidase 2
Omim ID
601870Gene Ontology
HyperlinkGene Summary
This gene is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2. [provided by RefSeq
Other Designations
eIF-2-associated p67 homolog|initiation factor 2-associated 67 kDa glycoprotein|peptidase M 2
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Interactome
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Disease
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Publication Reference
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Physiologically relevant metal cofactor for methionine aminopeptidase-2 is manganese.
Wang J, Sheppard GS, Lou P, Kawai M, Park C, Egan DA, Schneider A, Bouska J, Lesniewski R, Henkin J.
Biochemistry 2003 May; 42(17):5035.
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Negative regulation of the protection of eIF2alpha phosphorylation activity by a unique acidic domain present at the N-terminus of p67.
Datta R, Tammali R, Datta B.
Experimental Cell Research 2003 Feb; 283(2):237.
Application：WB, Rat, KRC-7 cells.
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Physiologically relevant metal cofactor for methionine aminopeptidase-2 is manganese.
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