The GGA1 polyclonal antibody (Cat # PAB4281) is used in Western blot to detect GGA1 in mouse kidney tissue lysate .
Western Blot (Cell lysate)
The GGA1 polyclonal antibody (Cat # PAB4281) is used in Western blot to detect GGA1 in Jurkat cell lysate .
A : N2a cells cotransfected with APP770-V5 and GGA1-myc or empty vector were immunostained for APP (Alexa488; green), GGA1 (Cy5; blue), and the Golgi marker GM130 (Cy3; red). B : The same transfection was stained for the endosomal marker EEA1 (Cy3). C : To assess cell-surface localization, APP695-GFP and GGA1-myc or empty vector were cotransfected and then immunostained with an Ab to the APP ectodomain on ice without permeabilization (Cy3). Cells were then fixed, permeabilized, and stained for GGA1 (Cy5). (J. Neurosci. 2006 Sep 27;26(39):9913-9922)
This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
ADP-ribosylation factor binding protein 1,OTTHUMP00000028975,OTTHUMP00000042200,gamma-adaptin related protein 1