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Last updated: 2016/12/11
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GGA1 polyclonal antibody

  • Catalog # : PAB4281
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of GGA1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human GGA1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:100-500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • The GGA1 polyclonal antibody (Cat # PAB4281) is used in Western blot to detect GGA1 in mouse kidney tissue lysate .
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The GGA1 polyclonal antibody (Cat # PAB4281) is used in Western blot to detect GGA1 in Jurkat cell lysate .
  • Immunofluorescence
  • Immunofluorescence
  • A : N2a cells cotransfected with APP770-V5 and GGA1-myc or empty vector were immunostained for APP (Alexa488; green), GGA1 (Cy5; blue), and the Golgi marker GM130 (Cy3; red).
    B : The same transfection was stained for the endosomal marker EEA1 (Cy3).
    C : To assess cell-surface localization, APP695-GFP and GGA1-myc or empty vector were cotransfected and then immunostained with an Ab to the APP ectodomain on ice without permeabilization (Cy3). Cells were then fixed, permeabilized, and stained for GGA1 (Cy5).
    (J. Neurosci. 2006 Sep 27;26(39):9913-9922)
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • GGA1
  • Gene Alias:
  • -
  • Gene Description:
  • golgi associated, gamma adaptin ear containing, ARF binding protein 1
  • Gene Summary:
  • This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • ADP-ribosylation factor binding protein 1,OTTHUMP00000028975,OTTHUMP00000042200,gamma-adaptin related protein 1
  • Gene Pathway
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