ARL3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of ARL3 polyclonal antibody (Cat # PAB4262) in A2058 cell line lysate (35 ug/lane). ARL3 (arrow) was detected using the purified ARL3 polyclonal antibody (Cat # PAB4262).
Western Blot (Transfected lysate)
Western blot analysis of ARL3 (arrow) using rabbit ARL3 polyclonal antibody (Cat # PAB4262). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ARL3 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with ARL3 polyclonal antibody (Cat # PAB4262) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ARL3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human ARL3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ARL3 polyclonal antibody (Cat # PAB4262) in A2058 cell line lysate (35 ug/lane). ARL3 (arrow) was detected using the purified ARL3 polyclonal antibody (Cat # PAB4262).Western Blot (Transfected lysate)
Western blot analysis of ARL3 (arrow) using rabbit ARL3 polyclonal antibody (Cat # PAB4262). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ARL3 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with ARL3 polyclonal antibody (Cat # PAB4262) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — ARL3
Entrez GeneID
403Protein Accession#
P36405;NP_004302Gene Name
ARL3
Gene Alias
ARFL3
Gene Description
ADP-ribosylation factor-like 3
Omim ID
604695Gene Ontology
HyperlinkGene Summary
ADP-ribosylation factor-like 3 is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. [provided by RefSeq
Other Designations
ARF-like 3|OTTHUMP00000020373
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Interactome
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Disease
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Publication Reference
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Assignment of the human ADP-ribosylation factor-like 3 (ARL3) gene to chromosome 10 band q23.3 by radiation hybrid mapping.
Kim HS.
Cytogenetics and Cell Genetics 1998 Dec; 83(3-4):246.
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Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence.
Adams MD, Kerlavage AR, Fleischmann RD, Fuldner RA, Bult CJ, Lee NH, Kirkness EF, Weinstock KG, Gocayne JD, White O, et al..
Nature 1995 Sep; 377(6547 Suppl):3.
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ADP-ribosylation factor (ARF)-like 3, a new member of the ARF family of GTP-binding proteins cloned from human and rat tissues.
Cavenagh MM, Breiner M, Schurmann A, Rosenwald AG, Terui T, Zhang C, Randazzo PA, Adams M, Joost HG, Kahn RA.
The Journal of Biological Chemistry 1994 Jul; 269(29):18937.
Application：WB-Ce, WB-Ti, Human, Mouse, HeLa, HL-60, H-226, H-345, MCF-7, NRK, PC-3 cells, Mouse brains, Recombinant protein.
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Assignment of the human ADP-ribosylation factor-like 3 (ARL3) gene to chromosome 10 band q23.3 by radiation hybrid mapping.
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