Western blot analysis of ARFGAP1 polyclonal antibody (Cat # PAB4255) against rat recombinant ARFGAP1 (30, 10, and 3 ng/lane, left to right) . ARFGAP1 (arrow) was detected using the purified ARFGAP1 polyclonal antibody (Cat # PAB4255) . Data courtesy of Dr . Dan Cassel, Department of Biology, Technion, Haifa, Israel .
Formalin-fixed and paraffin-embedded human cancer tissue reacted with ARFGAP1 polyclonal antibody (Cat # PAB4255) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq