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Last updated: 2016/10/23
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ARFGAP1 polyclonal antibody

  • Catalog # : PAB4255
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of ARFGAP1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human ARFGAP1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Rat
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:100-500)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of ARFGAP1 polyclonal antibody (Cat # PAB4255) against rat recombinant ARFGAP1 (30, 10, and 3 ng/lane, left to right) . ARFGAP1 (arrow) was detected using the purified ARFGAP1 polyclonal antibody (Cat # PAB4255) . Data courtesy of Dr . Dan Cassel, Department of Biology, Technion, Haifa, Israel .
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with ARFGAP1 polyclonal antibody (Cat # PAB4255) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Protein Accession#:
  • NP_060679;Q8N6T3
  • Gene Name:
  • Gene Alias:
  • ARF1GAP,HRIHFB2281,MGC39924
  • Gene Description:
  • ADP-ribosylation factor GTPase activating protein 1
  • Gene Summary:
  • The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • ADP-ribosylation factor 1 GTPase activating protein,ARF1-directed GTPase-activating protein,GAP protein,OTTHUMP00000031551
  • Gene Pathway
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