USP16 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of USP16 polyclonal antibody (Cat # PAB4204) in HL-60 cell line lysate (35 ug/lane). USP16 (arrow) was detected using the purified USP16 polyclonal antibody (Cat # PAB4204).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of USP16.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human USP16.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of USP16 polyclonal antibody (Cat # PAB4204) in HL-60 cell line lysate (35 ug/lane). USP16 (arrow) was detected using the purified USP16 polyclonal antibody (Cat # PAB4204).Enzyme-linked Immunoabsorbent Assay
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Gene Info — USP16
Entrez GeneID
10600Protein Accession#
UB16_HUMANGene Name
USP16
Gene Alias
UBP-M
Gene Description
ubiquitin specific peptidase 16
Omim ID
604735Gene Ontology
HyperlinkGene Summary
This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
OTTHUMP00000096468|deubiquitinating enzyme 16|ubiquitin carboxyl-terminal hydrolase 16|ubiquitin specific protease 16|ubiquitin thiolesterase 16|ubiquitin-specific processing protease 16
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Interactome
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Publication Reference
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Human and mouse proteases: a comparative genomic approach.
Puente XS, Sanchez LM, Overall CM, Lopez-Otin C.
Nature Reviews. Genetics 2003 Jul; 4(7):544.
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A mutant deubiquitinating enzyme (Ubp-M) associates with mitotic chromosomes and blocks cell division.
Cai SY, Babbitt RW, Marchesi VT.
PNAS 1999 Mar; 96(6):2828.
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Deubiquitinating enzymes: a new class of biological regulators.
D'Andrea A, Pellman D.
Critical Reviews in Biochemistry and Molecular Biology 1998 Jan; 33(5):337.
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Human and mouse proteases: a comparative genomic approach.
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