USP7 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of USP7.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human USP7.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The USP7 polyclonal antibody (Cat # PAB4186) is used in Western blot to detect USP7 in T-47D cell lysate .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the USP7 polyclonal antibody (Cat # PAB4186) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testis tissue reacted with USP7 polyclonal antibody (Cat # PAB4186) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .Enzyme-linked Immunoabsorbent Assay
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Gene Info — USP7
Entrez GeneID
7874Protein Accession#
UBP7_HUMANGene Name
USP7
Gene Alias
HAUSP, TEF1
Gene Description
ubiquitin specific peptidase 7 (herpes virus-associated)
Omim ID
602519Gene Ontology
HyperlinkOther Designations
Herpes virus-associated ubiquitin-specific protease|ubiquitin specific protease 7|ubiquitin specific protease 7 (herpes virus-associated)
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Interactome
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Disease
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Publication Reference
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Human and mouse proteases: a comparative genomic approach.
Puente XS, Sanchez LM, Overall CM, Lopez-Otin C.
Nature Reviews. Genetics 2003 Jul; 4(7):544.
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Deubiquitination of p53 by HAUSP is an important pathway for p53 stabilization.
Li M, Chen D, Shiloh A, Luo J, Nikolaev AY, Qin J, Gu W.
Nature 2002 Apr; 416(6881):648.
Application:IP, Human, H460 cells.
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Deubiquitinating enzymes: a new class of biological regulators.
D'Andrea A, Pellman D.
Critical Reviews in Biochemistry and Molecular Biology 1998 Jan; 33(5):337.
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Human and mouse proteases: a comparative genomic approach.
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