USP5 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of USP5.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 59-90 of human USP5.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50~100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The USP5 polyclonal antibody (Cat # PAB4182) is used in Western blot to detect USP5 in HL-60 cell lysate .Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — USP5
Entrez GeneID
8078Protein Accession#
P45974Gene Name
USP5
Gene Alias
ISOT
Gene Description
ubiquitin specific peptidase 5 (isopeptidase T)
Omim ID
601447Gene Ontology
HyperlinkOther Designations
ubiquitin isopeptidase T|ubiquitin specific peptidase 5|ubiquitin specific protease 5 (isopeptidase T)|ubiquitin-specific protease-5 (ubiquitin isopeptidase T)
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Interactome
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Publication Reference
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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA,
PNAS 2002 Dec; 99(26):16899.
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A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13.
Ansari-Lari MA, Muzny DM, Lu J, Lu F, Lilley CE, Spanos S, Malley T, Gibbs RA.
Genome Research 1996 Apr; 6(4):314.
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cDNA cloning of a human 100 kDa de-ubiquitinating enzyme: the 100 kDa human de-ubiquitinase belongs to the ubiquitin C-terminal hydrolase family 2 (UCH2).
Falquet L, Paquet N, Frutiger S, Hughes GJ, Hoang-Van K, Jaton JC.
FEBS Letters 1995 Dec; 376(3):233.
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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
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