DUSP14 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse bladder tissue lysate (35 ug/lane) with DUSP14 polyclonal antibody (Cat # PAB4142).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DUSP14 polyclonal antibody (Cat # PAB4142) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DUSP14.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human DUSP14.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse bladder tissue lysate (35 ug/lane) with DUSP14 polyclonal antibody (Cat # PAB4142).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DUSP14 polyclonal antibody (Cat # PAB4142) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DUSP14
Entrez GeneID
11072Protein Accession#
NP_008957;O95147Gene Name
DUSP14
Gene Alias
MKP-L, MKP6
Gene Description
dual specificity phosphatase 14
Omim ID
606618Gene Ontology
HyperlinkGene Summary
In addition to antigen recognition by the T-cell receptor, T-cell activation requires a second signal from a costimulatory receptor, such as CD28 (MIM 186760), which interacts with B7-1 (CD80; MIM 112203) and B7-2 (CD86; MIM 601020) ligands on antigen-presenting cells. CD28 costimulation induces transcription of interleukin-2 (IL2; MIM 147680) and stabilizes newly synthesized IL2 through the activation of mitogen-activated protein kinases (MAPKs), such as ERK (e.g., MAP2K4; MIM 601335) and JNK (see MIM 601158), and the subsequent creation of AP1 transcription factor (see MIM 165160). DUSP14 is a negative regulator of CD28 signaling.[supplied by OMIM
Other Designations
MKP-1 like protein tyrosine phosphatase|OTTHUMP00000164064|OTTHUMP00000164065
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Interactome
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Pathway
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Publication Reference
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Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6.
Marti F, Krause A, Post NH, Lyddane C, Dupont B, Sadelain M, King PD.
Journal of Immunology 2001 Jan; 166(1):197.
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Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6.
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