Western blot analysis of DUSP3 (arrow) using DUSP3 polyclonal antibody (Cat # PAB4127). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DUSP3 gene (Lane 2).
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DUSP3 polyclonal antibody (Cat # PAB4127) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq
serine/threonine specific protein phosphatase,vaccinia virus phosphatase VH1-related