TRPM8 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of TRPM8 (arrow) using rabbit TRPM8 polyclonal antibody (Cat # PAB4073). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRPM8 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinomareacted with TRPM8 polyclonal antibody (Cat # PAB4073), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TRPM8.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 933-963 at C-terminus (C940) of human TRPM8.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of TRPM8 (arrow) using rabbit TRPM8 polyclonal antibody (Cat # PAB4073). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRPM8 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinomareacted with TRPM8 polyclonal antibody (Cat # PAB4073), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TRPM8
Entrez GeneID
79054Protein Accession#
NP_076985;Q7Z2W7Gene Name
TRPM8
Gene Alias
LTRPC6, MGC2849, TRPP8
Gene Description
transient receptor potential cation channel, subfamily M, member 8
Omim ID
606678Gene Ontology
HyperlinkGene Summary
subfamily M
Other Designations
OTTHUMP00000065206|short form of the TRPM8 cationic channel|transient receptor potential subfamily M member 8
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Interactome
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Disease
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Publication Reference
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Characterisation of TRPM8 as a pharmacophore receptor.
Bodding M, Wissenbach U, Flockerzi V.
Cell Calcium 2007 Dec; 42(6):618.
Application：WB-Tr, Human, HEK 293 cells.
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Identification of an HLA-A*0201-restricted T-cell epitope derived from the prostate cancer-associated protein trp-p8.
Kiessling A, Fussel S, Schmitz M, Stevanovic S, Meye A, Weigle B, Klenk U, Wirth MP, Rieber EP.
Prostate 2003 Sep; 56(4):270.
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Trp-p8, a novel prostate-specific gene, is up-regulated in prostate cancer and other malignancies and shares high homology with transient receptor potential calcium channel proteins.
Tsavaler L, Shapero MH, Morkowski S, Laus R.
Cancer Research 2001 May; 61(9):3760.
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Characterisation of TRPM8 as a pharmacophore receptor.
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