PIK3CG polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of PIK3CG polyclonal antibody (Cat # PAB4069) in HeLa cell lysate (35 ug/lane). PIK3CG (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
Western Blot (Transfected lysate)
Western blot analysis of PIK3CG polyclonal antibody (Cat # PAB4069) in 293 cell lysate transiently transfected with the PIK3CG gene (2 ug/lane). PIK3CG (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human muscle tissue reacted with PIK3CG polyclonal antibody (Cat # PAB4069) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PIK3CG.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PIK3CG.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PIK3CG polyclonal antibody (Cat # PAB4069) in HeLa cell lysate (35 ug/lane). PIK3CG (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).Western Blot (Transfected lysate)
Western blot analysis of PIK3CG polyclonal antibody (Cat # PAB4069) in 293 cell lysate transiently transfected with the PIK3CG gene (2 ug/lane). PIK3CG (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human muscle tissue reacted with PIK3CG polyclonal antibody (Cat # PAB4069) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PIK3CG
Entrez GeneID
5294Protein Accession#
NP_002640;P48736Gene Name
PIK3CG
Gene Alias
PI3CG, PI3K, PI3Kgamma, PIK3
Gene Description
phosphoinositide-3-kinase, catalytic, gamma polypeptide
Omim ID
601232Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. [provided by RefSeq
Other Designations
1-phosphatidylinositol 3-kinase|PI3-kinase|PTDINS-3-kinase|p110-gamma|phosphatidylinositol 3 kinase gamma, p110 gamma|phosphatidylinositol 3-kinase catalytic 110-kD gamma|phosphatidylinositol 3-kinase, catalytic, gamma polypeptide|phosphoinositide-3-kinas
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Interactome
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Pathway
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Disease
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Publication Reference
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The IL-1 receptor accessory protein is essential for PI 3-kinase recruitment and activation.
Reddy SA, Lin YF, Huang HJ, Samanta AK, Liao WS.
Biochemical and Biophysical Research Communications 2004 Apr; 316(4):1022.
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Activation of the hexosamine pathway leads to phosphorylation of insulin receptor substrate-1 on Ser307 and Ser612 and impairs the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin insulin biosynthetic pathway in RIN pancreatic beta-cells.
Andreozzi F, D'Alessandris C, Federici M, Laratta E, Del Guerra S, Del Prato S, Marchetti P, Lauro R, Perticone F, Sesti G.
Endocrinology 2004 Mar; 145(6):2845.
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Inhibition of the PI3K-Akt signaling pathway enhances the sensitivity of Fas-mediated apoptosis in human gastric carcinoma cell line, MKN-45.
Osaki M, Kase S, Adachi K, Takeda A, Hashimoto K, Ito H.
Journal of Cancer Research and Clinical Oncology 2003 Nov; 130(1):8.
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The IL-1 receptor accessory protein is essential for PI 3-kinase recruitment and activation.
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