PLAU polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PLAU.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PLAU.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PLAU polyclonal antibody (Cat # PAB4042) in CEM cell lysate (35 ug/lane). PLAU (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PLAU polyclonal antibody (Cat # PAB4042) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PLAU
Entrez GeneID
5328Protein Accession#
P00749Gene Name
PLAU
Gene Alias
ATF, UPA, URK, UROKINASE, u-PA
Gene Description
plasminogen activator, urokinase
Gene Ontology
HyperlinkGene Summary
This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000019849|U-plasminogen activator|antagonist of uPA|plasminogen activator, urinary|urokinase plasminogen activator|urokinase-type plasminogen activator amino-terminal fragment
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Interactome
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Pathway
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Disease
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Publication Reference
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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA,
PNAS 2002 Dec; 99(26):16899.
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(4-aminomethyl)phenylguanidine derivatives as nonpeptidic highly selective inhibitors of human urokinase.
Sperl S, Jacob U, Arroyo de Prada N, Stürzebecher J, Wilhelm OG, Bode W, Magdolen V, Huber R, Moroder L.
PNAS 2000 May; 97(10):5113.
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Mutational analysis of the genes encoding urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 in advanced ovarian cancer.
Turkmen B, Schmitt M, Schmalfeldt B, Trommler P, Hell W, Creutzburg S, Graeff H, Magdolen V.
Electrophoresis 1997 May; 18(5):686.
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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
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