PFKFB4 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The PFKFB4 polyclonal antibody (Cat # PAB4031) is used in Western blot to detect PFKFB4 in mouse brain tissue lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PFKFB4 polyclonal antibody (Cat # PAB4031) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PFKFB4.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human PFKFB4.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The PFKFB4 polyclonal antibody (Cat # PAB4031) is used in Western blot to detect PFKFB4 in mouse brain tissue lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PFKFB4 polyclonal antibody (Cat # PAB4031) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PFKFB4
Entrez GeneID
5210Protein Accession#
Q16877Gene Name
PFKFB4
Gene Alias
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Gene Description
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
Omim ID
605320Gene Ontology
HyperlinkGene Summary
O
Other Designations
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-4|bifunctional enzyme with kinase and biphosphatase activities
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Interactome
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Pathway
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Publication Reference
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Hypoxic activation of PFKFB4 in breast tumor microenvironment shapes metabolic and cellular plasticity to accentuate metastatic competence.
Tao Dai, Spencer R Rosario, Eriko Katsuta, Abhisha Sawant Dessai, Emily J Paterson, Aaron T Novickis, Eduardo Cortes Gomez, Bokai Zhu, Song Liu, Hai Wang, Scott I Abrams, Mukund Seshadri, Wiam Bshara, Subhamoy Dasgupta.
Cell Reports 2022 Dec; 41(10):111756.
Application：WB-Ce, WB-Tr, Human, EMT6, MDA-MB-468 cells.
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Cloning, expression and chromosomal localization of a human testis 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene.
A Manzano, J X Perez, M Nadal, X Estivill, A Lange, R Bartrons.
Gene 1999 Mar; 229(1-2):83.
Application：WB-Tr, Monkey, COS-1 cells.
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Cloning of cDNA encoding for a novel isozyme of fructose 6-phosphate, 2-kinase/fructose 2,6-bisphosphatase from human placenta.
Sakai A, Kato M, Fukasawa M, Ishiguro M, Furuya E, Sakakibara R.
Journal of Biochemistry 1996 Mar; 119(3):506.
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Hypoxic activation of PFKFB4 in breast tumor microenvironment shapes metabolic and cellular plasticity to accentuate metastatic competence.
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