PFKFB1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of PFKFB1 polyclonal antibody (Cat # PAB4021) in Jurkat cell lysate (35 ug/lane). PFKFB1 (arrow) was detected using the purified polyclonal antibody (1 : 240 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with PFKFB1 polyclonal antibody (Cat # PAB4021) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PFKFB1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human PFKFB1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PFKFB1 polyclonal antibody (Cat # PAB4021) in Jurkat cell lysate (35 ug/lane). PFKFB1 (arrow) was detected using the purified polyclonal antibody (1 : 240 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with PFKFB1 polyclonal antibody (Cat # PAB4021) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PFKFB1
Entrez GeneID
5207Protein Accession#
F261_HUMANGene Name
PFKFB1
Gene Alias
F6PK, HL2K, MGC116715, MGC116717, PFRX
Gene Description
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Omim ID
311790Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. [provided by RefSeq
Other Designations
6PF-2-K/Fru-2,6-P2ASE liver isozyme|OTTHUMP00000023391|fructose-2,6-bisphosphatase|fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase
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Interactome
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Pathway
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Disease
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Publication Reference
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Sequence of human liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
Lange AJ, Pilkis SJ.
Nucleic Acids Research 1990 Jun; 18(12):3652.
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Molecular cloning, sequence analysis, and expression of a human liver cDNA coding for fructose-6-P,2-kinase:fructose-2,6-bisphosphatase.
Algaier J, Uyeda K.
Biochemical and Biophysical Research Communications 1988 May; 153(1):328.
Application：WB-Ti, Rat, Liver.
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Sequence of human liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
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