PFKFB1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PFKFB1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PFKFB1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of PFKFB1 polyclonal antibody (Cat # PAB4020) in Jurkat cell lysate (35 ug/lane). PFKFB1 (arrow) was detected using the purified polyclonal antibody (1 : 240 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with PFKFB1 polyclonal antibody (Cat # PAB4020) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — PFKFB1
Entrez GeneID
5207Protein Accession#
F261_HUMANGene Name
PFKFB1
Gene Alias
F6PK, HL2K, MGC116715, MGC116717, PFRX
Gene Description
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Omim ID
311790Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. [provided by RefSeq
Other Designations
6PF-2-K/Fru-2,6-P2ASE liver isozyme|OTTHUMP00000023391|fructose-2,6-bisphosphatase|fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Sequence of human liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
Lange AJ, Pilkis SJ.
Nucleic Acids Research 1990 Jun; 18(12):3652.
-
Molecular cloning, sequence analysis, and expression of a human liver cDNA coding for fructose-6-P,2-kinase:fructose-2,6-bisphosphatase.
Algaier J, Uyeda K.
Biochemical and Biophysical Research Communications 1988 May; 153(1):328.
Application:WB-Ti, Rat, Liver.
-
Sequence of human liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com