HK3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HK3 polyclonal antibody (Cat # PAB4002) in HeLa cell lysate (35 ug/lane). HK3 (arrow) was detected using the purified polyclonal antibody (1 : 40 dilution).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HK3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human HK3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HK3 polyclonal antibody (Cat # PAB4002) in HeLa cell lysate (35 ug/lane). HK3 (arrow) was detected using the purified polyclonal antibody (1 : 40 dilution).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HK3
Entrez GeneID
3101Protein Accession#
NP_002106Gene Name
HK3
Gene Alias
HKIII, HXK3
Gene Description
hexokinase 3 (white cell)
Omim ID
142570Gene Ontology
HyperlinkGene Summary
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq
Other Designations
ATP:D-hexose 6-phosphotransferase|hexokinase 3
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Interactome
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Pathway
- Amino sugar and nucleotide sugar metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
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Publication Reference
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Interaction of insulin-like growth factor binding protein-4, Miz-1, leptin, lipocalin-type prostaglandin D synthase, and granulin precursor with the N-terminal half of type III hexokinase.
Sui D, Wilson JE.
Archives of Biochemistry and Biophysics 2000 Oct; 382(2):262.
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Hexokinase isoenzymes in normal and cirrhotic human liver: suppression of glucokinase in cirrhosis.
Lowes W, Walker M, Alberti KG, Agius L.
Biochimica et Biophysica Acta 1998 Jan; 1379(1):134.
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Sequence of human hexokinase III cDNA and assignment of the human hexokinase III gene (HK3) to chromosome band 5q35.2 by fluorescence in situ hybridization.
Furuta H, Nishi S, Le Beau MM, Fernald AA, Yano H, Bell GI.
Genomics 1996 Aug; 36(1):206.
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Interaction of insulin-like growth factor binding protein-4, Miz-1, leptin, lipocalin-type prostaglandin D synthase, and granulin precursor with the N-terminal half of type III hexokinase.
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