AK2 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
The AK2 polyclonal antibody (Cat # PAB3993) is used in Western blot to detect AK2 in mouse kidney tissue lysate.
Western Blot (Cell lysate)
Western blot analysis of AK2 polyclonal antibody (Cat # PAB3993) in CEM cell lysate (35 ug/lane). AK2 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with AK2 polyclonal antibody (Cat # PAB3993) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AK2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human AK2.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
The AK2 polyclonal antibody (Cat # PAB3993) is used in Western blot to detect AK2 in mouse kidney tissue lysate.Western Blot (Cell lysate)
Western blot analysis of AK2 polyclonal antibody (Cat # PAB3993) in CEM cell lysate (35 ug/lane). AK2 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with AK2 polyclonal antibody (Cat # PAB3993) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — AK2
Entrez GeneID
204Protein Accession#
P54819Gene Name
AK2
Gene Alias
ADK2
Gene Description
adenylate kinase 2
Omim ID
103020Gene Ontology
HyperlinkGene Summary
Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
ATP-AMP transphosphorylase|OTTHUMP00000004287|OTTHUMP00000004288|adenylate kinase isoenzyme 2, mitochondrial|adenylate kinase, mitochondrial
-
Interactome
-
Pathway
-
Publication Reference
-
Cloning and expression of human adenylate kinase 2 isozymes: differential expression of adenylate kinase 1 and 2 in human muscle tissues.
Lee Y, Kim JW, Lee SM, Kim HJ, Lee KS, Park C, Choe IS.
Journal of Biochemistry 1998 Jan; 123(1):47.
Application：WB-Ti, Human, Skeletal muscle, Heart.
-
cDNA cloning and tissue-specific expression of the gene encoding human adenylate kinase isozyme 2.
Noma T, Song S, Yoon YS, Tanaka S, Nakazawa A.
Biochimica et Biophysica Acta. 1998 Jan; 1395(1):34.
-
Cloning and characterization of cDNA for human adenylate kinase 2A.
Lee Y, Kim JW, Lee IA, Kang HB, Choe YK, Lee HG, Lim JS, Kim HJ, Park C, Choe IS.
Biochemistry and Molecular Biology International 1996 Jul; 39(4):833.
-
Cloning and expression of human adenylate kinase 2 isozymes: differential expression of adenylate kinase 1 and 2 in human muscle tissues.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com