AK3L1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AK3L1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human AK3L1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of AK3L1 polyclonal antibody (Cat # PAB3989) in mouse kidney tissue lysate (35 ug/lane). AK3L1 (arrow) was detected using the purified polyclonal antibody (1 : 50dilution).Enzyme-linked Immunoabsorbent Assay
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Gene Info — AK3L1
Entrez GeneID
205Protein Accession#
NP_037542;NP_001005353;NP_982289;P27144Gene Name
AK3L1
Gene Alias
AK3, AK4, MGC166959
Gene Description
adenylate kinase 3-like 1
Omim ID
103030Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq
Other Designations
ATP-AMP transphosphorylase|GTP:AMP phosphotransferase|OTTHUMP00000010594|mitochondrial adenylate kinase-3|nucleoside-triphosphate-adenylate kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Structure and expression of human mitochondrial adenylate kinase targeted to the mitochondrial matrix.
Noma T, Fujisawa K, Yamashiro Y, Shinohara M, Nakazawa A, Gondo T, Ishihara T, Yoshinobu K.
The Biochemical Journal 2001 Aug; 358(Pt 1):225.
Application:WB-Ce, WB-Ti, WB-Tr, Human, Mouse, Brains, Hearts, HEK 293 cells, Kidneys, Livers, Skeletal muscles.
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Identification of a novel human adenylate kinase. cDNA cloning, expression analysis, chromosome localization and characterization of the recombinant protein.
Van Rompay AR, Johansson M, Karlsson A.
European Journal of Biochemistry 1999 Apr; 261(2):509.
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Identification of a novel adenylate kinase system in the brain: cloning of the fourth adenylate kinase.
Yoneda T, Sato M, Maeda M, Takagi H.
Brain Research. Molecular Brain Research 1998 Nov; 62(2):187.
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Structure and expression of human mitochondrial adenylate kinase targeted to the mitochondrial matrix.
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