Calmodulin polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Calmodulin.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human Calmodulin.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The Calmodulin polyclonal antibody (Cat # PAB3927) is used in Western blot to detect Calmodulin in Ramos cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with Calmodulin polyclonal antibody (Cat # PAB3927) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. -
Publication Reference
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Calmodulin and HIV type 1: interactions with Gag and Gag products.
Radding W, Williams JP, McKenna MA, Tummala R, Hunter E, Tytler EM, McDonald JM.
AIDS Research and Human Retroviruses 2000 Oct; 16(15):1519.
Application:IF, Human, H-9 cells.
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Calmodulin regulation of basal and agonist-stimulated G protein coupling by the mu-opioid receptor (OP(3)) in morphine-pretreated cell.
Wang D, Surratt CK, Sadee W.
Journal of Neurochemistry 2000 Aug; 75(2):763.
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Characterization of the human CALM2 calmodulin gene and comparison of the transcriptional activity of CALM1, CALM2 and CALM3.
Toutenhoofd SL, Foletti D, Wicki R, Rhyner JA, Garcia F, Tolon R, Strehler EE.
Cell Calcium 1998 May; 23(5):323.
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Calmodulin and HIV type 1: interactions with Gag and Gag products.
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