UBE2H polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of UBE2H (arrow) using UBE2H polyclonal antibody (Cat # PAB3899).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2H gene (Lane 2).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of UBE2H.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human UBE2H.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of UBE2H (arrow) using UBE2H polyclonal antibody (Cat # PAB3899).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2H gene (Lane 2). -
Gene Info — UBE2H
Entrez GeneID
7328Protein Accession#
UBCH_HUMANGene Name
UBE2H
Gene Alias
E2-20K, UBC8, UBCH, UBCH2
Gene Description
ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)
Omim ID
601082Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq
Other Designations
ubiquitin carrier protein H|ubiquitin-conjugating enzyme E2H|ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8)|ubiquitin-protein ligase H
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Interactome
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Pathway
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Disease
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Publication Reference
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Construction of a physical and transcript map flanking the imprinted MEST/PEG1 region at 7q32.
Hayashida S, Yamasaki K, Asada Y, Soeda E, Niikawa N, Kishino T.
Genomics 2000 Jun; 66(2):221.
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A human ubiquitin-conjugating enzyme homologous to yeast UBC8.
Kaiser P, Seufert W, Hofferer L, Kofler B, Sachsenmaier C, Herzog H, Jentsch S, Schweiger M, Schneider R.
The Journal of Biological Chemistry 1994 Mar; 269(12):8797.
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Construction of a physical and transcript map flanking the imprinted MEST/PEG1 region at 7q32.
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