UBE4A polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of UBE4A polyclonal antibody (Cat # PAB3891) in NCI-H460 and CEM cell line lysates (35 ug/lane). UBE4A (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of UBE4A.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human UBE4A.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of UBE4A polyclonal antibody (Cat # PAB3891) in NCI-H460 and CEM cell line lysates (35 ug/lane). UBE4A (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).Immunohistochemistry
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Gene Info — UBE4A
Entrez GeneID
9354Protein Accession#
UB4A_HUMANGene Name
UBE4A
Gene Alias
E4, KIAA0126, MGC133315, UBOX2, UFD2
Gene Description
ubiquitination factor E4A (UFD2 homolog, yeast)
Omim ID
603753Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. [provided by RefSeq
Other Designations
homologous to yeast UFD2|ubiquitin conjugation factor E4 A|ubiquitination factor E4A|ubiquitination factor E4A (homologous to yeast UFD2)
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Interactome
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Pathway
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Publication Reference
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Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1.
Nagase T, Seki N, Tanaka A, Ishikawa K, Nomura N.
DNA Research 1995 Aug; 2(4):167.
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Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1.
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