THY1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of THY1 polyclonal antibody (Cat # PAB3877) pre-incubated without (lane 1) and with (lane 2) blocking peptide in T-47D cell line lysate. THY1 (N-term) (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
Western Blot (Cell lysate)
The THY1 polyclonal antibody (Cat # PAB3877) is used in Western blot to detect THY1 in HL-60 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with THY1 polyclonal antibody (Cat # PAB3877) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of THY1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human THY1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of THY1 polyclonal antibody (Cat # PAB3877) pre-incubated without (lane 1) and with (lane 2) blocking peptide in T-47D cell line lysate. THY1 (N-term) (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).Western Blot (Cell lysate)
The THY1 polyclonal antibody (Cat # PAB3877) is used in Western blot to detect THY1 in HL-60 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with THY1 polyclonal antibody (Cat # PAB3877) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — THY1
Entrez GeneID
7070Protein Accession#
NP_006279;P04216Gene Name
THY1
Gene Alias
CD90, FLJ33325
Gene Description
Thy-1 cell surface antigen
Omim ID
188230Gene Ontology
HyperlinkOther Designations
Thy-1 T-cell antigen|Thy-1 membrane glycoprotein
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Interactome
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Pathway
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Disease
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Publication Reference
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Thy-1 expression in human fibroblast subsets defines myofibroblastic or lipofibroblastic phenotypes.
Koumas L, Smith TJ, Feldon S, Blumberg N, Phipps RP.
The American Journal of Pathology 2003 Oct; 163(4):1291.
Application：IF, Human, Human parental myometrial and orbital fibroblast strains.
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THY1 expression is associated with tumor suppression of human ovarian cancer.
Abeysinghe HR, Cao Q, Xu J, Pollock S, Veyberman Y, Guckert NL, Keng P, Wang N.
Cancer Genetics and Cytogenetics 2003 Jun; 143(2):125.
Application：Flow Cyt, Human, Caov-3, GR, HOC8, MLS/P, OVCAR-3, OW-1, SK-OV-3 cells.
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The Thy-1/Thy-1 ligand interaction is involved in binding of melanoma cells to activated Thy-1- positive microvascular endothelial cells.
Saalbach A, Hildebrandt G, Haustein UF, Anderegg U.
Microvascular Research 2002 Jul; 64(1):86.
Application：Flow Cyt, IHC-Fr, Human, Melanoma, HDMECs.
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Thy-1 expression in human fibroblast subsets defines myofibroblastic or lipofibroblastic phenotypes.
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