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PDHX polyclonal antibody

  • Catalog # : PAB3861
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PDHX.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to residues surrounding T11 of human PDHX.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • Immunofluorescence (1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of PDHX polyclonal antibody (Cat # PAB3861) in NCI-H460 cell line lysates (35 ug/lane).PDHX (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with PDHX polyclonal antibody (Cat # PAB3861), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Immunofluorescence
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Gene Information
  • Entrez GeneID:
  • 8050
  • Protein Accession#:
  • NP_003468;O00330
  • Gene Name:
  • PDHX
  • Gene Alias:
  • DLDBP,E3BP,OPDX,PDX1,proX
  • Gene Description:
  • pyruvate dehydrogenase complex, component X
  • Gene Summary:
  • The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex, see MIM 300502. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kD protein called protein X. Component X binds to the E3 (MIM 238331) component of the PDH complex (Robinson et al., 1990 [PubMed 2112155]; Aral et al., 1997 [PubMed 9399911]).[supplied by OMIM
  • Other Designations:
  • E3-binding protein,pyruvate dehydrogenase complex, lipoyl-containing component X
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