The PDHX polyclonal antibody (Cat # PAB3860) is used in Western blot to detect PDHX in mouse heart tissue lysate.
Western Blot (Transfected lysate)
Western blot analysis of PDX1 (arrow) using rabbit PDHX polyclonal antibody (Cat # PAB3860). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PDHX polyclonal antibody (Cat # PAB3860) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex, see MIM 300502. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kD protein called protein X. Component X binds to the E3 (MIM 238331) component of the PDH complex (Robinson et al., 1990 [PubMed 2112155]; Aral et al., 1997 [PubMed 9399911]).[supplied by OMIM
E3-binding protein,pyruvate dehydrogenase complex, lipoyl-containing component X