PDHX polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The PDHX polyclonal antibody (Cat # PAB3860) is used in Western blot to detect PDHX in mouse heart tissue lysate.
Western Blot (Transfected lysate)
Western blot analysis of PDX1 (arrow) using rabbit PDHX polyclonal antibody (Cat # PAB3860). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PDHX polyclonal antibody (Cat # PAB3860) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PDHX.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PDHX.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The PDHX polyclonal antibody (Cat # PAB3860) is used in Western blot to detect PDHX in mouse heart tissue lysate.Western Blot (Transfected lysate)
Western blot analysis of PDX1 (arrow) using rabbit PDHX polyclonal antibody (Cat # PAB3860). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PDHX polyclonal antibody (Cat # PAB3860) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. -
Gene Info — PDHX
Entrez GeneID
8050Protein Accession#
NP_003468;O00330Gene Name
PDHX
Gene Alias
DLDBP, E3BP, OPDX, PDX1, proX
Gene Description
pyruvate dehydrogenase complex, component X
Gene Ontology
HyperlinkGene Summary
The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex, see MIM 300502. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kD protein called protein X. Component X binds to the E3 (MIM 238331) component of the PDH complex (Robinson et al., 1990 [PubMed 2112155]; Aral et al., 1997 [PubMed 9399911]).[supplied by OMIM
Other Designations
E3-binding protein|pyruvate dehydrogenase complex, lipoyl-containing component X
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Interactome
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Publication Reference
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Quantitative proteomics: the copy number of pyruvate dehydrogenase is more than 10(2)-fold lower than that of complex III in human mitochondria.
Murray J, Gilkerson R, Capaldi RA.
FEBS Letters 2002 Oct; 529(2-3):173.
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Detection of a homozygous four base pair deletion in the protein X gene in a case of pyruvate dehydrogenase complex deficiency.
Ling M, McEachern G, Seyda A, MacKay N, Scherer SW, Bratinova S, Beatty B, Giovannucci-Uzielli ML, Robinson BH.
Human Molecular Genetics 1998 Mar; 7(3):501.
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Dihydrolipoamide dehydrogenase-binding protein of the human pyruvate dehydrogenase complex. DNA-derived amino acid sequence, expression, and reconstitution of the pyruvate dehydrogenase complex.
Harris RA, Bowker-Kinley MM, Wu P, Jeng J, Popov KM.
The Journal of Biological Chemistry 1997 Aug; 272(32):19746.
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Quantitative proteomics: the copy number of pyruvate dehydrogenase is more than 10(2)-fold lower than that of complex III in human mitochondria.
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