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Last updated: 2016/12/4
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NEUROD1 polyclonal antibody

  • Catalog # : PAB3857
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of NEUROD1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human NEUROD1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Immunofluorescence (1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of HepG2 cell lysate (35 ug/lane) with NEUROD1 polyclonal antibody (Cat # PAB3857).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEUROD1 polyclonal antibody (Cat # PAB3857) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma
  • Immunofluorescence
  • Immunofluorescence
  • ES cells were transiently transfected with flag-tagged mouse NEUROD1.
    Fixed 24h post transfection.Stained for flag tag (red) to check that some cells express protein. Most protein was in nucleus but some was cytoplasmic. Stained with NEUROD1 polyclonal antibody (Cat # PAB3857) at 1:100. NEUROD1 polyclonal antibody (Cat # PAB3857) showed strong and clear staining with similar pattern to the flag staining.
    (Supplied by Sally Lowell, Edinburgh University)
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4760
  • Protein Accession#:
  • NP_002491;Q13562
  • Gene Name:
  • NEUROD1
  • Gene Alias:
  • BETA2,BHF-1,NEUROD,bHLHa3
  • Gene Description:
  • neurogenic differentiation 1
  • Gene Summary:
  • This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq
  • Other Designations:
  • basic helix-loop-helix transcription factor,beta-cell E-box transactivator 2,neurogenic differentiation factor 1,neurogenic helix-loop-helix protein NEUROD
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