MAP2 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of MAP2 (arrow) using MAP2 polyclonal antibody (Cat # PAB3854).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2 gene (Lane 2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAP2 polyclonal antibody (Cat # PAB3854) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAP2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MAP2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of MAP2 (arrow) using MAP2 polyclonal antibody (Cat # PAB3854).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2 gene (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAP2 polyclonal antibody (Cat # PAB3854) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. -
Gene Info — MAP2
Entrez GeneID
4133Protein Accession#
NP_114033;P11137Gene Name
MAP2
Gene Alias
DKFZp686I2148, MAP2A, MAP2B, MAP2C
Gene Description
microtubule-associated protein 2
Omim ID
157130Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000163916
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Interactome
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Disease
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Publication Reference
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Comparative distribution of tau phosphorylated at Ser262 in pre-tangles and tangles.
Lauckner J, Frey P, Geula C.
Neurobiology of Aging 2003 Oct; 24(6):767.
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Specific binding of dehydroepiandrosterone to the N terminus of the microtubule-associated protein MAP2.
Laurine E, Lafitte D, Gregoire C, Seree E, Loret E, Douillard S, Michel B, Briand C, Verdier JM.
The Journal of Biological Chemistry 2003 Aug; 278(32):29979.
Application：WB-Re, Recombinant protein.
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Diagnostic value of microtubule-associated protein-2 (MAP-2) for neuroendocrine neoplasms.
Liu Y, Saad RS, Shen SS, Silverman JF.
Advances in Anatomic Pathology 2003 Mar; 10(2):101.
Application：IHC, Human, Carcinoid tumor, Large cell neuroendocrine carcinomas of lung, Melanoma.
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Comparative distribution of tau phosphorylated at Ser262 in pre-tangles and tangles.
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