NT5E polyclonal antibody
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with NT5E polyclonal antibody (Cat # PAB3846) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NT5E.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human NT5E.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with NT5E polyclonal antibody (Cat # PAB3846) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma -
Gene Info — NT5E
Entrez GeneID
4907Protein Accession#
NP_002517Gene Name
NT5E
Gene Alias
CD73, E5NT, NT, NT5, NTE, eN, eNT
Gene Description
5'-nucleotidase, ecto (CD73)
Omim ID
129190Gene Ontology
HyperlinkGene Summary
Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM
Other Designations
5' nucleotidase (CD73)|5' nucleotidase, ecto|OTTHUMP00000016808|OTTHUMP00000040565|Purine 5-Prime-Nucleotidase|ecto-5'-nucleotidase
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Interactome
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Pathway
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Disease
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Publication Reference
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Role of extracellular nucleotide phosphohydrolysis in intestinal ischemia-reperfusion injury.
Hart ML, Henn M, Kohler D, Kloor D, Mittelbronn M, Gorzolla IC, Stahl GL, Eltzschig HK.
FASEB Journal 2008 Mar; 22(8):2784.
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A simplified method for the preparation of detergent-free lipid rafts.
Macdonald JL, Pike LJ.
Journal of Lipid Research 2005 Feb; 46(5):1061.
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Role of extracellular nucleotide phosphohydrolysis in intestinal ischemia-reperfusion injury.
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