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Last updated: 2018/1/21
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ACO2 polyclonal antibody

  • Catalog # : PAB3751
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of ACO2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to internal region of human ACO2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent (Pierce), containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma) ; PVDF membrane was incubated in primary Ab ACO2 polyclonal antibody (Cat # PAB3751). Solution : 1 : 1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 °C. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.
  • Western Blot
  • Western Blot
  • Western blot analysis of ACO2 polyclonal antibody (Cat # PAB3751) in mouse heart (left) and 293 (right) cell lysates (35 ug/lane). ACO2 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human heartreacted with ACO2 polyclonal antibody (Cat # PAB3751), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 50
  • Protein Accession#:
  • NP_001089;Q99798
  • Gene Name:
  • ACO2
  • Gene Alias:
  • ACONM,MGC20605,MGC33908
  • Gene Description:
  • aconitase 2, mitochondrial
  • Gene Summary:
  • The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000042146,OTTHUMP00000165920,aconitase 2,aconitate hydratase,citrate hydro-lyase
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