UPF1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of UPF1 (arrow) using UPF1 polyclonal antibody (Cat # PAB3694).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UPF1 gene (Lane 2).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of UPF1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 7-37 of human UPF1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
ELISA
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of UPF1 (arrow) using UPF1 polyclonal antibody (Cat # PAB3694).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UPF1 gene (Lane 2).Enzyme-linked Immunoabsorbent Assay
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Gene Info — UPF1
Entrez GeneID
5976Protein Accession#
NP_002902;Q92900Gene Name
UPF1
Gene Alias
FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, RENT1, pNORF1
Gene Description
UPF1 regulator of nonsense transcripts homolog (yeast)
Omim ID
601430Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq
Other Designations
UP Frameshift 1|delta helicase|nonsense mRNA reducing factor 1|regulator of nonsense transcripts 1|up-frameshift mutation 1 homolog|yeast Upf1p homolog
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Interactome
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Publication Reference
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Phosphorylation of hUPF1 induces formation of mRNA surveillance complexes containing hSMG-5 and hSMG-7.
Ohnishi T, Yamashita A, Kashima I, Schell T, Anders KR, Grimson A, Hachiya T, Hentze MW, Anderson P, Ohno S.
Molecular Cell 2003 Nov; 12(5):1187.
Application：WB, Human, HeLa cells.
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Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay.
Lykke-Andersen J.
Molecular and Cellular Biology 2002 Dec; 22(23):8114.
Application：WB-Tr, Human, HEK 293 cells.
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Identification of delta helicase as the bovine homolog of HUPF1: demonstration of an interaction with the third subunit of DNA polymerase delta.
Carastro LM, Tan CK, Selg M, Jack HM, So AG, Downey KM.
Nucleic Acids Research 2002 May; 30(10):2232.
Application：WB-Ce, Human, HeLa cells.
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Phosphorylation of hUPF1 induces formation of mRNA surveillance complexes containing hSMG-5 and hSMG-7.
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