Western blot analysis of UPF1 (arrow) using UPF1 polyclonal antibody (Cat # PAB3694). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UPF1 gene (Lane 2).
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq
UP Frameshift 1,delta helicase,nonsense mRNA reducing factor 1,regulator of nonsense transcripts 1,up-frameshift mutation 1 homolog,yeast Upf1p homolog