HGF polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HGF.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 521-554 at C-terminus of human HGF.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Saturated Ammonium Sulfate (SAS) precipitation
Recommend Usage
Flow Cytometry (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The HGF polyclonal antibody (Cat # PAB3683) is used in Western blot to detect HGF in Ramos cell lysate. HGF (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with HGF polyclonal antibody (Cat # PAB3683) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.Flow Cytometry
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Gene Info — HGF
Entrez GeneID
3082Protein Accession#
NP_000592; P14210Gene Name
HGF
Gene Alias
F-TCF, HGFB, HPTA, SF
Gene Description
hepatocyte growth factor (hepapoietin A; scatter factor)
Omim ID
142409Gene Ontology
HyperlinkGene Summary
Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
fibroblast-derived tumor cytotoxic factor|hepatocyte growth factor|hepatopoietin A|lung fibroblast-derived mitogen|scatter factor
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Interactome
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Pathway
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Disease
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Publication Reference
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Co-expression of hepatocyte growth factor (HGF) and c-Met predicts peritoneal dissemination established by autocrine HGF/c-Met signaling in gastric cancer.
Toiyama Y, Yasuda H, Saigusa S, Matushita K, Fujikawa H, Tanaka K, Mohri Y, Inoue Y, Ajay G, Kusunoki M.
International Journal of Cancer 2012 Jun; 130(12):2912.
Application:IHC-P, Human, Human gastric cancer.
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Myocardial protective effect of human recombinant hepatocyte growth factor for prolonged heart graft preservation in rats.
Ryugo M, Sawa Y, Ono M, Fukushima N, Aleshin AN, Mizuno S, Nakamura T, Matsuda H.
Transplantation 2004 Oct; 78(8):1153.
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Autocrine expression of hepatocyte growth factor and its cytoprotective effect on hepatocyte poisoning.
He Y, Zhou J, Dou KF, Chen Y, Yan QG, Li HM.
World Journal of Gastroenterology 2004 Oct; 10(19):2827.
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The interactions of hepatocyte growth factor/scatter factor and its NK1 and NK2 variants with glycosaminoglycans using a modified gel mobility shift assay. Elucidation of the minimal size of binding and activatory oligosaccharides.
Lyon M, Deakin JA, Lietha D, Gherardi E, Gallagher JT.
The Journal of Biological Chemistry 2004 Oct; 279(42):43560.
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Co-expression of hepatocyte growth factor (HGF) and c-Met predicts peritoneal dissemination established by autocrine HGF/c-Met signaling in gastric cancer.
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