PDGFA polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PDGFA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PDGFA.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The PDGFA polyclonal antibody (Cat # PAB3678) is used in Western blot to detect PDGFA in HL-60 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with PDGFA polyclonal antibody (Cat # PAB3678) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — PDGFA
Entrez GeneID
5154Protein Accession#
PDGA_HUMANGene Name
PDGFA
Gene Alias
PDGF-A, PDGF1
Gene Description
platelet-derived growth factor alpha polypeptide
Omim ID
173430Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq
Other Designations
PDGF A-chain|platelet-derived growth factor alpha|platelet-derived growth factor alpha chain|platelet-derived growth factor alpha isoform 2 preproprotein
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Interactome
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Pathway
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Disease
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Publication Reference
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Phosphorylation of the carboxyl-terminal transactivation domain of c-Fos by extracellular signal-regulated kinase mediates the transcriptional activation of AP-1 and cellular transformation induced by platelet-derived growth factor.
Monje P, Marinissen MJ, Gutkind JS.
Molecular and Cellular Biology 2003 Oct; 23(19):7030.
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Platelet-derived growth factor induces the beta-gamma-secretase-mediated cleavage of Alzheimer's amyloid precursor protein through a Src-Rac-dependent pathway.
Gianni D, Zambrano N, Bimonte M, Minopoli G, Mercken L, Talamo F, Scaloni A, Russo T.
The Journal of Biological Chemistry 2003 Mar; 278(11):9290.
Application:WB, Human, HeLaAG cells.
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Kinetics control preferential heterodimer formation of platelet-derived growth factor from unfolded A- and B-chains.
Muller C, Richter S, Rinas U.
The Journal of Biological Chemistry 2003 May; 278(20):18330.
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Phosphorylation of the carboxyl-terminal transactivation domain of c-Fos by extracellular signal-regulated kinase mediates the transcriptional activation of AP-1 and cellular transformation induced by platelet-derived growth factor.
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